Journal: Genes & Diseases

Article Title: Suppression of osteosarcoma progression by engineered lymphocyte-derived proteomes

doi: 10.1016/j.gendis.2022.08.007

Figure Lengend Snippet: Suppression of tumorigenic behaviors of U2OS and MG63 OS cells by D-cAMP/cAMP- treated Jurkat cell-derived CM. CN = control, D-cAMP = Dibutyryl cAMP, CW = CW008, and CM = conditioned medium. The double asterisk indicates P < 0.01. (A, B) Suppression of MTT-based viability of U2OS and MG63 OS cells by D-cAMP-treated Jurkat cell-derived CM, respectively. (C – E) Suppression of MTT-based viability, EdU-based proliferation, and transwell invasion of U2OS cells by cAMP-treated Jurkat cell-derived CM. (F, G) Suppression of MTT-based viability of U2OS and MG63 OS cells by CW008-treated primary human T lymphocyte-derived serum-free CM, respectively.

Article Snippet: Primary human T lymphocytes (#33002-02, Celprogen, CA, USA) were cultured in CTS™ OpTmizer™ T-Cell Expansion SFM (#A3705001, Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Derivative Assay

Journal: Immunity

Article Title: OX40 Ligand Contributes to Human Lupus Pathogenesis by Promoting T follicular Helper Response

doi: 10.1016/j.immuni.2015.05.012

Figure Lengend Snippet: (A) Tfh gene expression by naïve and memory Th cells (from three donors) activated with anti-CD3 and anti-CD28 in the presence or absence of sOX40L for 48 h. Transcript counts in the cultured Th cells are shown after normalization to housekeeping genes. Mean ± s.d., n=3. Paired t-test. * p <0.05, ** p <0.01.

Article Snippet: Th cells were stimulated overnight with CD3/CD28 Dynabeads (Invitrogen) in RPMI complete medium supplemented with 10% FCS.

Techniques: Expressing, Cell Culture

Journal: Immunity

Article Title: OX40 Ligand Contributes to Human Lupus Pathogenesis by Promoting T follicular Helper Response

doi: 10.1016/j.immuni.2015.05.012

Figure Lengend Snippet: (A) Expression of CXCR5, IL-21, CD40L, Bcl-6, and T-bet by naïve and memory Th cells activated with anti-CD3 and anti-CD28 in the presence or absence of sOX40L and/or IL-12. Gated to FSChiSSChi activated cells. A representative result out of 3 independent experiments is shown.

Article Snippet: Th cells were stimulated overnight with CD3/CD28 Dynabeads (Invitrogen) in RPMI complete medium supplemented with 10% FCS.

Techniques: Expressing

Journal: Immunity

Article Title: OX40 Ligand Contributes to Human Lupus Pathogenesis by Promoting T follicular Helper Response

doi: 10.1016/j.immuni.2015.05.012

Figure Lengend Snippet: (A) Bcl-6 expression by naïve Th cells transfected with the indicated siRNA and cultured for 3 days with anti-CD3 and CD28 ± sOX40L. Gated to FSChiSSChi activated cells. Mean ± s.e.m., n=3.

Article Snippet: Th cells were stimulated overnight with CD3/CD28 Dynabeads (Invitrogen) in RPMI complete medium supplemented with 10% FCS.

Techniques: Expressing, Transfection, Cell Culture